Abstract
(1) Isolated fat cells from rat epididymal fat pad when incubated in vitro in medium containing 32P i , showed a time-dependent increase in specific radioactivity of microsomal phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol; incubation of fat cells in the presence of adrenaline resulted in an increased specific radioactivity only of phosphatidylcholine compared with phospholipids in incubated control cells. (2) Investigation of the incorporation of 32P i into the water-soluble precursors of phosphatidylcholine showed that the increase in radioactivity of phosphorylcholine was time-dependent, and was similar in both control and adrenaline-stimulated fat cells. Incorporation of 32P i into CDP-choline was less than into phosphorylcholine, and was markedly increased in fat cells incubated with adrenaline when compared with controls. (3) The concentration of phosphorylcholine in fat cells has been determined. (4) The fractional turnover rate of phosphatidylcholine has been calculated, and was found to be increased in adrenaline-stimulated cells when compared with incubated controls.
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