The effect of hyperosmotic treatment on the functioning of ampullary electroreceptor organs.

Abstract

The condition of the microvilli of the apical membrane in ampullary electroreceptor organs of the freshwater catfish, Ictalurus nebulosus, was manipulated by administration of hyperosmotic solutions (500 mM) in order to investigate to what extent the apical surface area determines the sensitivity and the shape of the frequency curves. Electrophysiological recordings demonstrated that hyperosmotic sucrose and glucose solutions reduce the sensitivity by 80%, and alter the shape of the frequency curves. The synaptic delay and the average spontaneous activity remained almost unchanged. Calculations with an equivalent electric circuit of an electroreceptor cell indicate that a reduction in apical surface area can account for these effects. Apparently, the sensitivity and the frequency curves in ampullary organs in freshwater catfish depend on the apical surface area. A hyperosmotic urea solution proved to be less suitable to investigate the effect of manipulations of the apical surface area. The almost total loss in sensitivity caused by administration of urea is caused by both shunting of the stimulus by leaky tight junctions and a reduced synaptic efficacy. The repetitive activity observed after exposure to glucose or sucrose must be attributed to malfunctioning of postsynaptic parts of the receptor organ.