Abstract
The main aim of the study is to examine the effect of sodium hydrosulfide (NaHS), an H2S donor, on the oxidative stress in human plasma in vitro. It also examined the effects of very high concentrations of exogenous hydrogen sulfide on the hemostatic parameters (coagulation and fibrinolytic activity) of human plasma. Plasma was incubated for 5–30 min with different concentrations of NaHS from 0.01 to 10 mM. Following this, lipid peroxidation was measured as a thiobarbituric acid reactive substance (TBARS) concentration and the oxidation of amino acid residues in proteins was measured by determining the amounts of thiol groups and carbonyl groups. Hydrogen peroxide (H2O2) and the hydroxyl radical generating oxidation system (Fe/H2O2) were used as oxidative stress inducers. Hemostatic factors, such as the maximum velocity of clot formation, fibrin lysis half-time, the activated partial thromboplastin time (APTT), thrombin time (TT), and international normalized ratio (INR), were estimated. Changes in lipid peroxidation, carbonyl group formation, and thiol group oxidation were detected at high concentrations of H2S (0.1–10 mM), and these results indicate that NaHS (as the precursor of H2S) may have pro-oxidative effects in human plasma in vitro. Moreover, considering the data presented in this study, we suggest that the oxidative stress stimulated by NaHS (at high concentrations: 1–10 mM) is not involved in changes of the hemostatic activity of plasma.
Highlights
Hydrogen sulfide (H2 S) is a well-known toxic gas synthesized from the amino acids l- and d-cysteine (Cys) and l-homocysteine (l-Hcy)
We examined the amidolytic activity of plasmin in human plasma, and its influence on other hemostatic parameters: the activated partial thromboplastin time (APTT), thrombin time (TT), and international normalized ratio (INR) of human plasma in vitro
NaHS that was added to human plasma in vitro at concentrations of 0.1–10 mM induced lipid peroxidation after 5, 15, and 30 min of incubation (Table 1)
Summary
Hydrogen sulfide (H2 S) is a well-known toxic gas synthesized from the amino acids l- and d-cysteine (Cys) and l-homocysteine (l-Hcy). Zaicho et al [10] classified modulators of H2 S metabolism into three groups: (1) agents that reduce the amount of hydrogen sulfide in tissues (specific and nonspecific inhibitors of H2 S synthesis),. (2) agents with an uncertain impact (some medicines), and (3) agents that increase the amount of hydrogen sulfide (inorganic and organic H2 S donors). Sodium hydrosulfide (NaHS) and sodium sulfide (Na2 S) were the first H2 S donors to be studied in the cardiovascular system [11]. Na2 S is currently under evaluation in phase I and II trials as a therapeutic agent
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