The effect of HSP70/CD80 DNA vaccine on airway inflammation by regulating the chemokine and the receptor of chemokine in asthmatic mice

Abstract

Objective To determine the effect of HSP70/CD80 DNA vaccine on airway inflammation and airway hyperresponsiveness in asthma mice through regulating Th1/Th2 balance in the chemokine and its receptor.Methods Mouse model of acute asthma was established by OVA-sensitized.At the same time,mice were injected with HSP70/CD80 DNA vaccine for observing airway responsiveness (AR),detecting serum IgE,measuring the pathological changes of lung tissue,and detecting CCL5 and CCL17 expression.The expression levels of CCR5 and CCR4 mRNA in lung tissue were examined using real-time PCR.Results Compared with the control mice,mice with acute asthma showed a significant increase in airway reactivity and the infiltration of inflammatory cells in lung tissue.Mice treated with HSP70/CD80 DNA vaccine could effectively reduce airway hyperresponsiveness in airway,as well as the perivascular inflammatory response (P＜0.05).The expression of CCL5 was positive in airway epithelia,while CCL17 was negative (P＜0.05).CCR5 gene expression in lung tissue was increased,while CCR4 expression was decreased (P＜0.05).Conclusion HSP70/CD80 DNA vaccine could treat asthma through restoring Th1/Th2 balance in two regulation ways,of which one was by enhancing the expression of CCL5 in airway epithelial cell and reducing CCL17 expression,the other was up-regulating the ratio of CCR5/CCR4 via increasing the expression of CCR5 and decreasing the expression of CCR4. Key words: Asthma; Airway inflammation; CCR4; CCR5; CCL5; CCL17