Abstract
PurposeTo find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th.Method128 three-week-old weaned rats underwent high-fat diet and exercise (60–70% VO2max, 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were detected by ELISA, RT-qPCR, and Western blotting.Result(1) The protein of KISS-1 and GPR54 increased gradually during the growing period. KISS-1 mRNA peaked at 35D and GPR54 peaked at 43D. (2) High-fat diet affected the expression of the KISS-1/GPR54 system in rat testis and reduced the expression level of KISS-1 protein. (3) 60–70% VO2max exercise decreased the KISS-1/GPR54 expression level. Exercise intervention improved testicular development in rats with a high-fat diet.ConclusionThe expression of KISS-1/GPR54 increased during the growing period. High-fat diet can downregulate the protein and gene expression of KISS-1/GPR54 and change the expression trend. 60–70% VO2max exercise decreased the expression of KISS-1/GPR54, which may be involved in the effects of exercise on high-fat dietary sex hormone disorders.
Highlights
The discovery of the KISS-1/ G protein-coupled receptor 54, GPR 54 system has provided a new way to understand the link between energy and reproduction
The expression of KISS-1/G protein-coupled receptor 54 (GPR54) increased during the growing period
High-fat diet can downregulate the protein and gene expression of KISS-1/GPR54 and change the expression trend. 60–70% VO2max exercise decreased the expression of KISS-1/GPR54, which may be involved in the effects of exercise on high-fat dietary sex hormone disorders
Summary
The discovery of the KISS-1/ G protein-coupled receptor 54, GPR 54 system has provided a new way to understand the link between energy and reproduction. Studies have shown that the axons of KISS-1 neurons attach to GnRH neurons and GPR54 neurons that are located on GnRH neurons. The KISS-1/ GPR54 system is recognized for its ability to control. Our previous studies found that high-fat diet intervention could upregulate the expression of KISS-1/GPR54 in the hypothalamus of growing male rats, but it could lead to lipid droplet deposition in testicular tissue and decrease the serum testosterone level. According to its gene expression profile, KISS-1 is mostly highly expressed in the brain, followed by tissues such as placenta, testis, pancreas, liver, and small intestine; GPR54 is highly expressed in the brain, followed by tissues such as pancreas, placenta, and testis [2]. The function of the KISS-1/GPR54 system in peripheral tissues is still unclear [3,4,5,6,7]
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