Effects Of Exercise On The Expression Of Kiss-1/GPR54 In Testis Of High-fat Diet Rats In Growth Phase

Abstract

PURPOSE: To explore the effect of exercise on localization and expression of KISS-1 and GPR54 in the growth period (PND 21st to 56th day) of high-fat diet rat testes. METHODS: 21D old weanling rats were randomly divided into group HC (n=32) and group HE (n=32). HE group took 5-weeks trained (60-70% vVO2max, 1h/day, 5days/week). Groups HC and HE were fed with high-fat feed, which was purchased from Beijing Huafukang Biotechnology Co. Ltd. (LOT No: D12451).The rats of two groups were killed on the 21st D, 35th D, 43rd D, and 56th D old. The localization, mRNA expression and protein expression of KISS-1/GPR54 in the testis of each group were tested. RESULTS: The high-fat diet intervention resulted in a decrease in testicular index from pre-puberty(p<0.05); testicular tissue testosterone decreased significantly from prepuberty(p<0.01), and endocrine function was impaired; rat testicular tissue structure was loose, and the number of mature sperm decreased. The high-fat diet intervention had no significant effect on the localization of KISS-1/GPR54 system in rat testis, but affected the expression of KISS-1/GPR54 in rat testis and down-regulated the expression of KISS-1 protein(p<0.01). Exercise intervention can improve testicular development in rats with high-fat diet, especially to improve sex hormone disorders caused by high-fat diet. Under exercise intervention, the testicular tissue testosterone decline caused by high-fat diet was improved(p<0.05). Comparing with HC group, the expression of GPR54 protein increased significantly(p<0.01),but the KISS-1 protein expression had no obvious change(p>0.05). CONCLUSION: High-fat diet can inhibit the testicular development of male rats in the growth phase, and can also down-regulate the protein and gene expression levels of KISS-1/GPR54 system in testis tissue, and change the expression of KISS-1/GPR54 system, and its role needs further study. 60~70% VO2max moderate-intensity aerobic exercise change the inhibitory effect of high-fat diet on testicular development in male rats, and up-regulate KISS-1/GPR54 in prepubertal stage and whether KISS-1/GPR54 in testicular tissue participates in its regulation remains to be further study.