Abstract
The effect of different doses of low intensity laser therapy (L.I.L.T.) on human fibroblasts was investigated to determine the optimal dose required to stimulate fibroblast proliferation. Human fibroblasts were cultured in vitro and irradiated with different energy densities of 83Onm continuous output infra-red laser using a Gallium Aluminium Arsenide laser. The fibroblasts were irradiated on three consecutive days at energy densities, ranging from 0.2 to 5 J.cm2, delivered at an average radiant power of 30 mW, and at a constant distance of lcm from the fibroblasts. Fibroblast activity was assessed on the fourth day using a calorimetric MTT (tetrazolium) cleavage assay. There was a significant increase in fibroblast proliferation at laser treatment energy densities of 0.4 J.cm2 and 5 J.cm2. Difficulties associated with in vivo and in vitro studies of the effect of laser treatment are discussed.
Highlights
Fibroblasts were cultured in vitro and irradiated with different energy densities of 83Onm continuous output infra-red laser using a Gallium Aluminium Arsenide laser
High energy lasers provide discretely focused and localised thermal energy to coagulate or vaporise tissue, while low energy “athermic” lasers are used in low intensity laser therapy (L.I.L.T.) which has become a popular form of non-invasive treatment to pro mote wound healing. (Basford, 1989)
The aim of this study was to investi gate the effect of infra-red Ga-Al-As laser therapy on a component of the wound healing process, human fibroblast activity, and to determine the optimal dose required to stimulate fibroblast activity in vitro
Summary
Arsen id eAnL aIns eVritroonDFosiibmreotrbylaStsutdAy c tiv it y : ABSTRACT: The effect of different doses of low intensity laser ther apy (L.I.L.T.) on humanfibroblasts was investigated to determine the optimal dose required to stimulate fibroblast proliferation. There was a significant increase infibroblast proliferation at laser treatment energy densities of 0.4. High energy lasers provide discretely focused and localised thermal energy to coagulate or vaporise tissue, while low energy “athermic” lasers are used in low intensity laser therapy (L.I.L.T.) which has become a popular form of non-invasive treatment to pro mote wound healing. Low intensity laser therapy is thought to provide a biostimulatory effect, enhancing the natural process of wound healing (Brom, 1994). Despite a large body of in vitro and in vivo research on the use of LILT, the clinician is faced with the problem that there is no general ly agreed or scientifically validated dose for laser therapy in humans (Enwemeka, 1988) and the dose related efficacy of correspondence
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