The Effect of GA3 Concentration, Micro Tubers Size, and Dark or Light Storage on Breaking Potato Dormancy

Abstract

Potatoes (Solanum tuberosum L) are included in priority crops considering their function as a source of carbohydrates in food diversification. One of the techniques in producing quality seed is through tissue culture continued within in vitro tuber production that is micro tubers. The aim of this research was to observe the effect of GA3 concentration, storage, and size of micro tubers on breaking dormancy. The activities were carried out in the tissue culture laboratory of IVEGRI from April to July 2018. The treatments were to determine the effect of GA3 (10,15,20 ppm) and the size of micro tubers cv. Granola i.e. large (>4 g); small (<1 g); medium (>1 g - <4 g), and storage system dark (G), light (T). The experimental design was a complete randomized block design with 3 replications, each treatment consists of 20 micro tubers. Results of experiment micro tubers size and storage were significantly different, GA3 concentration does not affect the percentage shoot growth which initializes the breaking dormancy of tubers at 8 to 10 WAS (Weeks After Storage). Average shoot number 0.3 – 1.25 per tuber and shoot length 0.30 – 0.80 cm. In general, each micro tuber only grows one shoot. On visual observation, the large size of micro tubers will have quality and length of shoot better than small size micro tubers.