Abstract
Refrigerated preservation is a simple and inexpensive method for the short-term preservation of fish sperm used in the hatchery. The type of extender is an important factor that determines the nature of the preservation conditions because certain extenders are better suited for preserving sperm obtained from specific species. Therefore, this study aims to determine the best type of extender for the refrigerated preservation of sperm obtained from climbing perch (Anabas testudineus). The study was conducted using three replications of five types of extenders, which include Alsever's solution, urea solution, Ringer solution, physiological NaCl and glucose-base. Subsequently, the sperm was diluted with each extender at a sperm-extender dilution rate (v/v) of 1:60, stored at 4°C and assayed every 24 h for 6 days to determine the sperm quality. The results showed that the type of extender had a significant effect on the sperm motility, viability and fertilization rates of climbing perch after refrigerated preservation (p < 0.05). Also, the highest values of motility, viability and fertilization rates from the sperm diluted with glucose-base were 45.83%, 43.83% and 46.28%, respectively, which were significantly different from other treatments. Therefore, the glucose-base was the best extender for the refrigerated preservation of climbing perch (A. testudineus) sperm in comparison to others extender used.
Published Version
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