The Effect of Ethanolic Extract of Black and White Rice Bran (Oryza sativa L.) on Cancer Cells

Abstract

Indonesia has a wide range of rice cultivars and pigments. This rice can be used as a source ofphytochemical compounds for cancer prevention. This research aims to analyze the cytotoxic activitiesof the ethanolic extract of black rice bran of 4 local cultivars i.e. ‘Cempo Ireng’, ‘Woja Laka’, ‘Toraja’ and‘IR­64’ (white rice) on cancer cells and to determine the compounds groups of those extracts. First step,rice bran was extracted with ethanol. This extract was applied to Raji (a human Burkitt Lymphomacancer), HepG2 (a human liver cancer), and Vero (a nonhuman cell line) cells in order to measure thecytotoxic activities by using MTT assay. To determine descriptively the compounds groups of phenolics,flavonoids, terpenoids, steroids, and alkaloids the thin layer chromatography method was performed.The IC50 value was analyzed quantitatively by using probit analysis. Results showed that the IC50 valuesof ethanolic extract of rice bran ‘Woja Laka’, ‘Toraja’, ‘Cempo Ireng’ and ‘IR 64’ on HepG2 cells were857.23±99.19; 1,896.55±83,8; 1,494.47±87.81 and 727.89±145,97 µg/ml respectively. The IC50 on Raji cellswere 816.61±85.31; 1,079.93±28.31; 1,627.82; ±119.82, and 769.33±61.43 µg/ml respectively. The IC50 onVero cells were 1,295.2±37; 1,232.07±165.51; 1,874.14±169.56, and 724.4±122.79 µg/ml respectively. Theethanolic extracts of rice bran from four cultivars contain phenolics, flavonoids, terpenoids, and steroids.However, alkaloids could not be detected. The variety of rice cultivars indicates the variation of cytotoxicactivities on cancer cells. The ethanolic extracts of rice bran from those four rice cultivars contain similarkinds of organic compounds groups but vary in the Rf values.