The effect of elemene in combination with vascular endothelial growth factor polyclonal antibody on the expression of proliferating cell nuclear antigen and tissue inhibitor of metalloproteinase-1 in brain glioma tissue of rats

Abstract

Objective To investigate the effect of elemene in combination with vascular endothelial growth factor (VEGF) polyclonal antibody on the expression of proliferating cell nuclear antigen (PCNA) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in brain glioma tissue of rats. Methods The rat glioma models were built by intracerebral injection of C6 glioma cells. Forty-eight successful rat models were selected and randomly divided into model group, elemene group, VEGF polyclonal antibody group and elemene in combination with VEGF polyclonal antibody group, with 12 rats in each group. Rats in model group were performed intraperitoneal injection of 0.5 ml normal saline, rats in elemene group were performed intraperitoneal injection of 0.5 ml elemene injection, rats in VEGF polyclonal antibody group were performed intraperitoneal injection of 0.5 ml VEGF polyclonal antibody, and rats in elemene in combination with VEGF polyclonal antibody group were performed intraperitoneal injection of 0.5 ml elemene injection and 0.5 ml VEGF polyclonal antibody. The drug was administered on alternate days for 7 consecutive times. The tumour volume, tumor inhibition rate and the expression of PCNA and TIMP-1 in brain tissue of the rats between the groups were compared. Statistics with SPSS 21.0 software, measurement data for Mean±SD indicated that one factor variance analysis was used for the comparison between the two groups, and t test was used for the comparison between the two groups. Results (1) Tumor volume and tumor inhibition rate: The tumor volumes in model group, elemene group, polyclonal group, VEGF polyclonal antibody group and elemene in combination with VEGF polyclonal antibody group were (68.54±8.12), (37.65±6.24), (39.12±6.41), (30.12±5.24) mm3, respectively; the tumor inhibition rates in elemene group, VEGF polyclonal antibody group and elemene in combination with VEGF polyclonal antibody group were (445.07±6.45)%, (42.92±6.52)%, (56.05±7.21)%, respectively. The tumor volume in elemene in combination with VEGF polyclonal antibody group was significantly smaller than that in control group (t=10.449, 9.851, 13.772, P<0.01); the tumor volume in elemene in combination with VEGF polyclonal antibody group was significantly smaller than that in elemene group and VEGF polyclonal antibody group (t=3.201, 3.766, P<0.05), and the tumor inhibition rate was significantly higher than that in elemene group and VEGF polyclonal antibody group (t=3.932, 4.679, P<0.05). (2) As to the expression of PCNA and TIMP-1 in brain tissue: the PCNA indexes of tumors in model group, elemene group, VEGF polyclonal antibody group and elemene in combination with VEGF polyclonal antibody group were (73.12±10.21, 44.24±6.42, 47.36±7.12, 37.45±5.24), respectively, and the TIMP-1 indexes were (45.24±6.45, 62.45±8.15, 59.45±7.32, 78.65±11.24), respectively. The PCNA indexes of tumors in elemene group, VEGF polyclonal antibody group and elemene in combination with VEGF polyclonal antibody group were significantly lower than that in model group (t=8.295, 7.447, 10.767, P<0.05, P<0.01), the TIMP-1 indexes were significantly higher than that in model group (t=5.736, 5.401, 8.931, P<0.05, P<0.01); the PCNA index of tumor in elemene in combination with VEGF polyclonal antibody group was significantly lower than that in elemene group and VEGF polyclonal antibody group (t=2.838, 3.491, P<0.05), and the TIMP-1 index was significantly higher than that in elemene group and VEGF polyclonal antibody group (t=4.042, 4.700, P<0.05). Conclusion Elemene in combination with VEGF polyclonal antibody can help to inhibit the growth of brain glioma tissue, which may be related to the regulation of PCNA and the expression of TIMP-1 in brain tissue. Key words: Brain glioma; Elemene; Vascular endothelial growth factor polyclonal antibody; Proliferating cell nuclear antigen; Tissue inhibitor of metalloproteinase-1