The effect of drought stress on the expression of genes TaMOR, TaNAC69-1 and TaExpb23 in different bread wheat (Triticum aestivum L.) cultivars

Abstract

For evaluating the effects of TaMOR, TaNAC69-1 and TaExpb23 expression on root system architecture and drought tolerance of wheat, 10 bread wheat varieties were compared through germination test, pot experiment and field trial. In germination test, genotypes were evaluated under 0, -3 and -6 bar osmotic potentials. In pot experiment genotypes were compared at 50%, 75% and 100% of field capacity (FC) irrigation regime. In field trial, genotypes were grown under well-watered and rain fed conditions. Based on the results of this experiment, it was concluded that water deficit had significant effect on all of traits except for seminal root numbers. Significant genotypic differences were observed for all of traits indicating the presence of genetic variability among the genotypes. Correlation coefficients showed that TaMOR gene expression had positive correlation with plant yield under 75%FC stress (r=0.733*). TaNAC69-1 gene expression had positive correlations with plant yield and plant biomass under 50%FC (r=0.634* and r=0.711*). TaNAC69-1 gene expression also showed positive correlation with root numbers under 50%FC condition (r=0.622*). TaNAC69-1 gene expression also showed positive correlation with seminal root angle (r=0.648*) under severe stress. TaExpb23 expression showed positive correlation with plant yield and biomass under 75%FC (r=0.663* and r=0.723*). Based on the results of this experiment, it was concluded that under different stress levels, TaMOR, TaNAC69-1 and TaExpb23 gene expressions had significant correlations with traits. Due to significant G×E interactions, it also concluded that the effect of expression of TaMOR, TaNAC69-1 and TaExpb23 genes on traits is different under distinct stress levels.