Abstract
Fat body from blowfly larvae was incubated with a mixture of amino acids, [U- 14C] valine, and various Ringer's solutions. Incorporation of [U- 14C] valine into protein was considerably affected by changes in the level of calcium, magnesium, potassium, and sodium in the incubation medium. During 60 min incubation there was a fall in the levels of alanine, aspartate, glutamate, and phenylalanine in the medium and a rise in glutamine and tyrosine. An examination of the in vivo distribution of free amino acids between various tissues of the larva showed that aspartate and glutamate were concentrated in tissues other than the haemolymph and that phenylalanine and tyrosine were concentrated in the fat body in particular.
Published Version
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