Abstract
During in vitro multiplication of Hosta ‘Gold Drop’, 20 g l-1 sucrose, 5.5 g l-1 agar and 4 concentrations (0.1-0.8 ml l-1) of Ferbanat L, Kelpak, Pentakeep-V were added to half-strength Murashige and Skoog (MS) basal medium. As compared to the control and other biostimulators, plants with lower peroxidase activity, larger fresh weight, more, longer shoots and roots, larger leaves were developed on medium containing Kelpak. The best concentration was 0.4 ml l-1 for in vitro rooting, shoot formation, plant weight and ex vitro chlorophyll, carotenoid level, peroxidase activity. Pentakeep was the less efficient biostimulator, increasing of its concentration mostly decreased root and shoot values (furthermore, abnormal callus formation was observed, as non-wanted effect), chlorophyll content and sizes (length, width) of leaves, not only during in vitro propagation but also (as after-effect) acclimatization because of the high mortality and weakly developed survivor plants.
Highlights
In order to eliminate harmful environmental effects, considerable limitations have been placed on several compounds applied in horticultural production, which consist synthetic growth regulators and plant protection chemicals (Ludwig-Muller, 2000)
If Pelargonium zonale ‘Serena’ cuttings were soaked or soaked + sprayed with 0.5% Pentakeep-V, significantly longer, more and heavier roots were formed, but no significant differences were obtained between the way of biostimulator application (Köbli et al, 2012)
20-35 g l-1 sucrose is recommended, to the cultivars (Szafián, 2010)
Summary
In order to eliminate harmful environmental effects, considerable limitations have been placed on several compounds applied in horticultural production, which consist synthetic growth regulators (e.g. auxins) and plant protection chemicals (Ludwig-Muller, 2000). Biostimulators contain different growth regulators or other hormonal components These natural, organic preparations often decrease stresses, increase physiological activity, accelerate root development and regularly applied in horticulture but rarely in plant propagation (Szabó et al, 2011). The same (and higher: 0.5 ml l-1) dose was optimal for Saintpaulia ionantha (Tilly-Mándy et al, 2010b), because treated plants produced flowers two weeks earlier than the control and developed larger leaf-rozettes with higher chlorophyll concentration. If Pelargonium zonale ‘Serena’ cuttings were soaked or soaked + sprayed with 0.5% Pentakeep-V, significantly longer, more and heavier roots were formed, but no significant differences were obtained between the way of biostimulator application (Köbli et al, 2012). With the use of Pentakeep-V during cut flower production of Lilium cultivars (Abay, 2014), higher chlorophyll content, better vegetative developments, enhanced CO2 assimilation were detected
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