In vitro induction of shoots and roots from Jatropha curcas L. explants

Abstract

SummaryAn appropriate in vitro multiple shoot induction medium was established for Jatropha curcas L. from various explants. Shoot regeneration from apical shoots, nodes, axillary bud-derived shoots, petioles and leaf explants were assessed on Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzyladenine (BA) alone, or in combination with indole-3-yl-butyric acid (IBA). MS medium with 2.22 – 4.44 µM BA was effective for apical shoot culture, while 4.44 µM BA was suitable for node culture. MS medium with 2.22 µM BA and 0.049 µM IBA provided the best shoot proliferation from axillary bud-derived shoots. For petiole segments from leaves at the second, third and fourth nodes, the most effective growth regulator combination for callus induction during the first culture cycle, and subsequent shoot formation during the second culture cycle, was MS medium supplemented with 4.44 µM BA and 2.46 µM IBA. A combination of 8.88 µM BA and 4.90 µM IBA was suitable for callus induction and shoot formation from leaf segments at the second and third nodes of branches. For rooting, 4.90 µM IBA induced 85.71% rooting from shoots on basal rooting medium. The in vitro plantlets were acclimatised and transferred to potting mix, with 82% survival.