THE EFFECT OF DIETARY CASEIN ON THE INDUCTION OF LUNG TUMOURS BY THE INJECTION OF 9,10-DIMETHYL-1,2-BENZANTHRACENE (DMBA) INTO NEWBORN MICE.

Margaret A Walters,F J C Roe

doi:10.1038/bjc.1964.36

Abstract

The Effect of Dietary Casein on the Induction of Lung Tumours by the Injection of 9,10-Dimethyl-1,2-Benzanthracene (DMBA) into Newborn Mice

Highlights

MATERIALS AND METHODSBALB/c (Bittner agent free) mice of a line originally obtainied from Dr H
It is concluded that DMBA induces significantly more lung tumours in mice on a high caseiin diet than in those on a low casein diet
In previous experiments the modification of carcinogenesis by changes in dietary protein has been unequivocally demonstrated in the case of liver tumours only

Summary

MATERIALS AND METHODS

BALB/c (Bittner agent free) mice of a line originally obtainied from Dr H. All animals were examined thoroughly once each week and more cursorily each day when they were fed. Group 1: high casein diet + DMBA. Groups 2 and 3: high casein diet controls combined. Groups 5 and 6: low casein diet controls combined. The level of casein in the low protein diet was reduced from 15 % to 10 %. (In a previous trial in which a 10 % protein diet was fed to lactating mothers, a large proportion of the sucklings died before weaning, mainly through cannibalism.) The body weights of mice in treated and control groups fed high and low protein diets were similar throughout the experiment (Fig. 1).

Results of Experiment

DISCUSSION

SUMMARY