Abstract
Objective: The aim of this study was to evaluate the effect of dexmedetomidine (100µg/kg-ip) on ischemia reperfusion (IR) injury in myocard of rats. Methods: Twenty-four Wistar Albino rats were separated into four groups. There were four experimental groups (Group C (Control; n=6), Group IR (ischemia-reperfusion, n=6), Group D (Dexmedetomidine; n=6), underwent left thoracotomy and received ip dexmedetomidine without ischemia and reperfusion and Group IR-D (IR-Dexmedetomidine; n=6) was administrated 100µg/kg dexmedetomidine via ip route 30 minutes before ligating the left coronary artery. A small plastic snare was threaded through the ligature and placed in contact with the heart. To produce IR, a branch of the left coronary artery was occluded for 30 min followed by two hours of reperfusion. However, after the above procedure, the coronary artery was not occluded or reperfused in the control rats. At the end of the study, myocard tissue was obtained for biochemical, histochemical and immunohistochemical determination/ analyses . Results: Myonecrosis, cell infiltration and edema were significantly higher in the IR group than in the C and D groups. In the IR-D group, myonecrosis, cell infiltration and edema were significantly lower than in the IR group.TBARS levels were found to be significantly higher in the IR group than in the C and D groups.TBARSlevels in the IR-D group were found to be significantly lower than in the IR group.SOD enzyme activity was found to be significantly lower in the IR group than in the C group. In the IR-D group, SOD enzyme activity was found to be significantly higherthan the IR group. Conclusion: Dexmedetomidine removed degenerative effects after ischemia reperfusion in ischemia reperfusion group and we may conclude that dexmedetomidine may have regenerative effects on IR injury .
Highlights
Ischemia is defined as the significant reduction of blood flow and the insufficiency of oxygen and nutrients’ provision to the various tissues and organs
In the IR-D group, myonecrosis, cell infiltration and edema were significantly lower than in the IR group.Thiobarbituric acid reactive substance (TBARS) levels were found to be significantly higher in the IR group than in the C and D groups.TBARSlevels in the IR-D group were found to be significantly lower than in the IR group.Superoxide Dismutase (SOD) enzyme activity was found to be significantly lower in the IR group than in the C group
One major contributing factor is the inability to protect the heart against the detrimental effects of lethal myocardial reperfusion injury, which occur on restoring blood flow to the acutely ischemic myocardium
Summary
Ischemia is defined as the significant reduction of blood flow and the insufficiency of oxygen and nutrients’ provision to the various tissues and organs. One major contributing factor is the inability to protect the heart against the detrimental effects of lethal myocardial reperfusion injury, which occur on restoring blood flow to the acutely ischemic myocardium. In this study it was demostrated that in ischemic rat hearts, oxygen related enzym release has an important role. Toxic injury during ischemic in myocart or the other cells, increses with tissue reperfusion. Animals were provided ad libitum access to standard rat chow and water and were allowed a minimum of 5 d to acclimate to the facility prior to any manipulation The protocols of this experimental study were approved by the Animal Ethics Committee of Gazi University (30.11.2011, G.U.ET-11.103). Statistical significance was set at a p value of
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