The Effect of deinoxanthin Isolated from Radioactivity-Resistant Bacteria on Expression of Bcl-2 Gene in Hela Cell Line

Abstract

Background: After breast cancer, uterine cervical neoplasms is the most common cancer in women. It is believed that genetic factors are effective in developing cancer. Bcl-2 is a well-known anti-apoptosis gene that increases cell viability without stimulating effect on cellular proliferation. Today it attempts to use natural compounds to control or treat diseases. Carotenoids are one of these compounds. Deinoxanthin is a carotenoid isolated from Deinococcus radiodurans. Since this bacterium has a unique ability to withstand radiation, and radiation is a well-known cause of cancer carotenoid synthesized by bacterium is worthwhile. The aim of study is evaluating the effect of deinoxanthin on the expression of Bcl-2 in Hela cell line. Methods: Active culture of bacteria was purchased from Genetic and Biological Reserve of Iran then deinoxanthin was extracted. Hela was prepared of Pasteur Institute of Iran and cultured. Cells were divided into two treatment and control groups. Deinoxanthin was affected on the treatment group and its toxicity was measured using MTT. Real-time ­PCR was used to measure gene expression. RNA was extracted from two groups and cDNA was made. Results: Real-time PCR showed the anti-apoptotic expression of Bcl-2 decreased by 4/85 and given that p-value of 0/05 was (p-value=0) this decrease is significant. Conclusion: Regarding the results of Real-time PCR it can be concluded deinoxanthin extract has an inhibitory effect on the uterine cancer cell line has an inhibitory effect after 48 hours and the amount of anti-apoptotic expression of Bcl-2 has significantly decreased (p-value=0).