The effect of CXCL10 blockade in C protein-induced myositis

Abstract

Event Abstract Back to Event The effect of CXCL10 blockade in C protein-induced myositis Jinhyun Kim1, Jiyong Choi2, Sunghae Chang2, Sung-Hye Park2, Kichul Shin2, Hye Won Kim2, Hyejin Oh2, Myeong Jae Yoon2, Bon Seung Ku2, Eun Young Lee2, Eun Bong Lee2, Hiroshi Kawachi3, Hitoshi Kohsaka4 and Yeong-Wook Song2* 1 Chungnam National Univeristy Hospital, South Korea 2 Seoul National University College of Medicine, South Korea 3 Niigata University Graduate School of Medical and Dental Sciences, Department of Cell Biology, Institute of Nephrology, Japan 4 Tokyo Medical and Dental University, Department of Medicine and Rheumatology, Japan Background: CXCL10 (also called interferon-γ-inducible protein 10 [IP-10]) is a chemokine that plays a critical role in the infiltration of T cell in autoimmune diseases. CXCL10 is reported to be expressed in muscle tissue of polymyositis, thus we investigated the role of CXCL10 and the effect of CXCL10 blockade in C protein-induced myositis, an animal model of polymyositis. Methods: C protein-induced myositis model was induced with human skeletal C protein fragment in 8-week-old female C57BL/6 mice. Immunohistochemistry was performed to detect CXCL10 and CXCR3, its receptor. CXCR3 in mouse splenocyte was investigated by flow cytometry. Migration assay of mouse splenocyte was performed with 5 μm pore transwell system. Mice with C protein-induced myositis were treated with anti-CXCL10 antibody or control IgG 8 days after the induction of myositis and the muscle inflammation was assessed 3 week after the induction. Results: Immunohistochemistry showed the expression of CXCL10 and CXCR3 in the muscle of C protein-induced myositis. Flow cytometry demonstrated IFN-γ+ cells were increased among CXCR3+CD8+ T cells compared to CXCR3-CD8+ T cells (CXCR3+CD8+ T cell, 28.0 ± 4.2% vs. CXCR3-CD8+ T cell, 9.5 ± 1.5%, p = 0.016). Migration of splenocyte was increased in response to CXCL10 (chemotactic index=1.91±0.45). Treatment with anti-CXCL10 antibody (n=10) showed less inflammation score in muscles than treatment with control IgG (n=10; median [range], anti IP-10, 0.75 [0.25-2.00] vs. control IgG, 1.43 [1.125-4.25], p=0.045). Conclusion: CXCL10 was expressed in the inflammation of C protein-induced myositis model and its blockade suppressed inflammation in muscle. Keywords: Polymyositis, CXCL10, CXCR3, mouse model, chemokine Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: Kim J, Choi J, Chang S, Park S, Shin K, Kim H, Oh H, Yoon M, Ku B, Lee E, Lee E, Kawachi H, Kohsaka H and Song Y (2013). The effect of CXCL10 blockade in C protein-induced myositis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00747 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 18 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Yeong-Wook Song, Seoul National University College of Medicine, Seoul, South Korea, ysong@snu.ac.kr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Jinhyun Kim Jiyong Choi Sunghae Chang Sung-Hye Park Kichul Shin Hye Won Kim Hyejin Oh Myeong Jae Yoon Bon Seung Ku Eun Young Lee Eun Bong Lee Hiroshi Kawachi Hitoshi Kohsaka Yeong-Wook Song Google Jinhyun Kim Jiyong Choi Sunghae Chang Sung-Hye Park Kichul Shin Hye Won Kim Hyejin Oh Myeong Jae Yoon Bon Seung Ku Eun Young Lee Eun Bong Lee Hiroshi Kawachi Hitoshi Kohsaka Yeong-Wook Song Google Scholar Jinhyun Kim Jiyong Choi Sunghae Chang Sung-Hye Park Kichul Shin Hye Won Kim Hyejin Oh Myeong Jae Yoon Bon Seung Ku Eun Young Lee Eun Bong Lee Hiroshi Kawachi Hitoshi Kohsaka Yeong-Wook Song PubMed Jinhyun Kim Jiyong Choi Sunghae Chang Sung-Hye Park Kichul Shin Hye Won Kim Hyejin Oh Myeong Jae Yoon Bon Seung Ku Eun Young Lee Eun Bong Lee Hiroshi Kawachi Hitoshi Kohsaka Yeong-Wook Song Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.