Dividing and non-dividing CD27low and CD27high human memory B-cells are differently induced depending on the stimulus.

Abstract

Event Abstract Back to Event Dividing and non-dividing CD27low and CD27high human memory B-cells are differently induced depending on the stimulus. Antonio Clemente1*, Jaume Pons1, Nallibe Lanio1, Núria Matamoros1 and Joana M. Ferrer1* 1 Hospital Universitario Son Espases, Spain Protective antibody responses depend on efficient memory B-cells activation. Heterogeneity among responding memory B cells has been shown greater than previously appreciated. According to their proliferating status and intensity of CD27 expression, different subpopulations, endowed with distinct functional characteristics, have been described in memory B-cells stimulated with CpG-ODN and interleukins. OBJECTIVES To evaluate the proportion of dividing and non-dividing functionally distinct CD27High or CD27Low memory B-cells generated after stimulation with a surrogate T-dependent (CD40) or T-independent (TLR9) stimulus in the presence or absence of IL-21 and/or BCR signaling. METHODS B-cells from healthy donors were purified from PBMC by magnetic negative selection. CFSE-labelled B-cells were stimulated with anti-CD40 or CpG-ODN, with or without anti-IgM, in the presence or absence of IL-21. B-cell proliferation and phenotype were evaluated by flow cytometry. RESULTS We found that neither anti-CD40 nor anti-IgM alone or in combination induced proliferating memory B-cells. However, CpG-ODN alone induced proliferation, with a higher proportion of dividing CD27Low than CD27High memory B-cells. IL-21 induced equal proportions of non-dividing CD27Low or dividing CD27Low and CD27High on CD40-activated memory B-cells. In contrast, IL-21 increased dividing CD27High and markedly decreased dividing CD27Low on CpG-ODN-activated memory B-cells. Addition of anti-IgM consistently increased the proportion of dividing CD27Low on memory B-cells costimulated with either anti-CD40+IL-21 or CpG-ODN. CONCLUSIONS Distinct proportions of dividing and non-dividing CD27High/Low memory B-cells are generated depending on stimulus. Given the different functional capabilities of these subpopulations, this has to be taken into account when trying to bias immune responses. Acknowledgements This work has been supported by the Fondo de Investigación Sanitaria from the Spanish Government (grants FIS PI08/0362 and FIS PI11/0160). References Henn AD, Laski M, Yang H, Welle S, Qiu X, Miao H et al. Functionally distinct subpopulations of CpG-activated memory B cells. www.nature.com/Scientific Reports | 2 : 345 | DOI: 10.1038/srep00345. Keywords: memory B cells, TLR9, CD40, BCR signaling, IL-21 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: Clemente A, Pons J, Lanio N, Matamoros N and Ferrer JM (2013). Dividing and non-dividing CD27low and CD27high human memory B-cells are differently induced depending on the stimulus.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00079 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Antonio Clemente, Hospital Universitario Son Espases, Palma de Mallorca, Spain, antonio.clemente@ssib.es Dr. Joana M Ferrer, Hospital Universitario Son Espases, Palma de Mallorca, Spain, juanam.ferrer@ssib.es Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Antonio Clemente Jaume Pons Nallibe Lanio Núria Matamoros Joana M Ferrer Google Antonio Clemente Jaume Pons Nallibe Lanio Núria Matamoros Joana M Ferrer Google Scholar Antonio Clemente Jaume Pons Nallibe Lanio Núria Matamoros Joana M Ferrer PubMed Antonio Clemente Jaume Pons Nallibe Lanio Núria Matamoros Joana M Ferrer Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.