Abstract
ABSTRACT Previous studies by us indicated, via indirect means, that cortisol decreased the incorporation of leucine into the total protein of cultured human neutrophils. These experiments have been repeated, employing autoradiographic procedures, in order to corroborate our previous observations as well as to obtain information on the possible effect of cortisol on the incorporation of uridine into neutrophil RNA. The results presented suggest that cortisol promotes a decrease in the incorporation of uridine into human neutrophil nuclear and cytoplasmic RNA, whereas, only the synthesis of nuclear protein appears affected. A correlation of these results to the neutrocytosis promoted by the in vitro administration of cortisol to humans is made and discussed.
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