Abstract
The appearance of radioactivity in cytoplasmic RNA was investigated in the salivary glands of mice injected with uridine- 3H at various times after a single intraperitoneal administration of isoproterenol (IPR). The results showed that: (1) the minimum time required for the appearance of radioactivity in cytoplasmic ribosomal RNA was the same in unstimulated and stimulated glands; (2) the incorporation of uridine- 3H into cytoplasmic rRNA was much higher in stimulated than in control glands, at 8, 18, and 20 hours after IPR; (3) the increase in incorporation of uridine- 3H was essentially the same in free and membrane-bound ribosomes; (4) this increase was completely inhibited by inhibitors of IPR-stimulated DNA synthesis; and (5) the incorporation of uridine- 3H into polydisperse (6S–16S) cytoplasmic RNA increases sharply at 8 hours after IPR. These results indicate that, in the IPR-stimulated salivary glands of mice, gene activation becomes detectable only several hours after the administration of IPR.
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