The effect of Cocoa Fruits (Theobroma cacao L.) extracts on serum blood glucose levels in white rats (Rattusnovergicus)

Abstract

Checking blood glucose levels supports healthy lifestyle and pattern changes to avoid foods that contain high glucose, which can increase blood glucose levels. This study aims to analyze the administration of cocoa pod extract (Theobroma cacao L.) to describe serum blood glucose levels in white rats (Rattusnovergicus). This study used an RCT (randomized controlled trial) design involving five groups of cocoa pod extract samples through an evaporation process using a rotary vacuum evaporator after going through the maceration process for 3 x 24 hours divided into three concentrations of 50 mg/Kg body wt, 100 mg/Kg body wt, 150 mg/Kg body wt. Furthermore, serum blood glucose levels will be measured on the 3rd, 7th, and 14th day after administration of metformin and waste extract of cocoa pod shell (Theobroma cacao L.). Analysis of the research data used a paired t-test. The results showed that the average fasting blood glucose levels of K1 rats (Mus musculus) to the average fasting glucose levels in K2, K3, K4, and K5, respectively, were as follows: 0.037 (p <0.05); 0.000 (p < 0.05); 0.028 (p < 0.05); and 0.015 (p <0.05). This shows that the average fasting blood glucose level is significantly different (p <0.05). The average fasting blood glucose levels of K2 (metformin group) to the average glucose levels in K3 (extract group 50 mg/kg body wt), K4 (extract group 100 mg/kg body wt), and K5 (extract group 150 mg/kg body wt) respectively are as follows: 0.157 (p > 0.05); 1.000 (p > 0.05); and 0.995 (p > 0.05). This shows that the average blood glucose levels between K2, K3, K4, and K5 did not differ significantly (p <0.05) after measuring cocoa pod extract for two days. Based on the results of data analysis from this experimental study, it can be concluded that there is an effect of giving cocoa pod extract (Theobroma cacao L.) on fasting blood glucose levels of mice (Mus musculus) with type 2 diabetes mellitus hypercholesterolemia model. Keywords: Diabetes mellitus; Cacao; Blood Glucose; Rats