Abstract
Six normolipidemic and six hypertriglyceridemic subjects were studied. The investigations were conducted before and after the basal diet (cholesterol intake about 0.8 mmol/day) was replaced by a cholesterol-rich diet (cholesterol intake about 4 mmol/day). Irrespective of the type of diet, the combined formation of cholic acid (C) and chenodeoxycholic acid (CD) was about two times higher in the hyperlipoproteinemic (mostly type IV) than the normolipidemic subjects. With the cholesterol-rich diet, the total plasma cholesterol increased in all normolipidemic and in four hyperlipidemic patients. Although total bile acid formation remained constant, there were several indications that an augmented intake of dietary cholesterol influenced bile acid metabolism. The pool size of CD increased in all but one normolipidemic subject. This group also displayed a decrease in the C/CD ratio of the bile acids produced and in the C/CD ratio of the bile acids in duodenal bile. The latter finding was also encountered in the hyperlipoproteinemic patients. On the basis of these and other data, it is suggested that the pattern of the bile acids synthesized may roughly reflect the degree of hepatic cholesterogenesis. Cholesterol feeding had no consistent effects on the molar cholesterol concentration in duodenal bile.-Andersén, E., and K. Hellström. The effect of cholesterol feeding on bile acid kinetics and biliary lipids in normolipidemic and hypertriglyceridemic subjects.
Highlights
Six normolipidemic and six hypertriglyceridemic subjects were studied
In accordance with this observation, the biosynthesis of cholic acid showed a proportionally higher increase than that of chenodeoxycholic acid upon stimulation of hepatic cholesterogenesis by administration of a bile acid-sequestering agent both in normolipidemic subjects [2] and in patients with the type 11 lipoprotein pattern [3]. If these findings have a general implication, the inhibition of hepatic cholesterogenesis by cholesterol feeding [4] may influence the pattern of the two primary bile acids produced. This hypothesis was tested in the current study, in which the kinetics of cholic and chenodeoxycholic acids were studied in normo- and hyperlipidemic subjects before and during intake of a cholesterol-rich diet
The low density lipoprotein (LDL) fraction was separated from high density lipoprotein (HDL) by precipitation of the former with manganese heparin [8]
Summary
The subjects in the study comprised six normolipidemic female members of the hospital staff and six hyperlipidemic patients (Table 1). All subjects were informed of the nature, purpose, and risks involved in the study before giving their informed consent. For 2 weeks before and during the second study the fat content in the basal diet was reduced and isocalorically compensated for by the fat in five eggs, supplied as a plain omelette. With this procedure the daily cholesterol intake during the first and the second studies averaged 0.8 and 4.0 mmol, respectively, in the normolipidemic subjects. Body weight and the plasma lipid levels were measured repeatedly during the studies
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