The effect of biologically and chemically synthesized silver nanoparticles (AgNPs) on biofilm formation

Joanna Chojniak,Izabela Biedroń,Grażyna Płaza,Barbara Mendrek,B Kaźmierczak,K Piekarska,J Danielewicz,H Jouhara,M Kutyłowska

doi:10.1051/e3sconf/20172200029

Abstract

Bionanotechnology has emerged up as integration between biotechnology and nanotechnology for developing biosynthetic and environmental-friendly technology for synthesis of nanomaterials. Different types of nanomaterials like copper, zinc, titanium, magnesium, gold, and silver have applied in the various industries but silver nanoparticles have proved to be most effective against bacteria, viruses and eukaryotic microorganisms. The antimicrobial property of silver nanoparticles are widely known. Due to strong antibacterial property silver nanoparticles are used, e.g. in clothing, food industry, sunscreens, cosmetics and many household and environmental appliances. The aim of the study was to compare the effect of silver nanoparticles (AgNPs) synthesized biologically and chemically on the biofilm formation. The biofilm was formed by the bacteria isolated from the water supply network. The commonly used crystal violet assay (CV) was applied for biofilm analysis. In this study effect of biologically synthesized Ag-NPs on the biofilm formation was evaluated.

Highlights

Nanoparticles of metals are currently an area of intense research due to a wide variety of their potential applications in many areas, for example in biomedical, agricultural, environmental fields
The physical properties of AgNPs formed in bio- and chemical synthesis are discussed by Mendrek et al [3]
The reduction in biofilm formation was observed in 10 tested strains as a result of biologically synthesized AgNPs

Summary

Introduction

Nanoparticles of metals are currently an area of intense research due to a wide variety of their potential applications in many areas, for example in biomedical, agricultural, environmental fields. Nanoparticles have specific properties and chemical reactivity [6, 17, 19]. One of them is high surface area to volume. The antimicrobial activity of AgNPs seems to be a function of the surface area to effectively interact with microorganisms. Large surface area of nanoparticles enhances the interaction with microbes and results in a wide spectrum of antimicrobial activities. Silver nanoparticles (AgNPs) are the most popular material to using as the antimicrobial agent

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