Antibacterial and Antioxidant Activity of Biosynthesized Silver Nanoparticle using Cassia Occidentalis

Abstract

Nanotechnology is a fascinating field of research that has applications in various areas such as material science, medical science, life science, physical and chemical sciences. A study was conducted to determine the antioxidant and antimicrobial properties of silver nanoparticles. The silver nanoparticles were biosynthesized from the leaves of Cassia occidentalis. The antimicrobial effectiveness of the biosynthesized silver nanoparticles was tested against Staphylococcus aureus and Pseudomonas Stutzeri, and in vitro antioxidant activities were measured using DPPH, FRAP, and Hydrogen peroxide. The microorganisms were treated with 10 and 20 µg/mL of the silver nanoparticles and 20 µg/mL of ciprofloxacin for 72 hours. After incubation, the silver nanoparticles significantly inhibited the growth of Staphylococcus aureus (17.00 ± 2.50), which was higher than the inhibition observed with ciprofloxacin (15.00 ± 0.05). The zone of inhibition of Pseudomonas Stutzeri was 15.00 ± 3.00, which was inhibited by the silver nanoparticles compared to ciprofloxacin (22.00 ± 2.00). The silver nanoparticles also showed significant antioxidant activity compared to the standard ascorbic acid. In conclusion, the study found that the biosynthesized silver nanoparticles exhibited antimicrobial and antioxidant properties against the tested microorganisms.