The Effect of Bakiko (Spinach- Chitosan- Collagen) Membrane on Total Fibroblast in Burn Wound Grade II

Abstract

<pre><strong><em>Background:</em></strong><em> According to WHO data in 2016, every year, about 265,000 deaths occurred due to burns and 38% of burn patients got pathological scars due to delayed wound healing. Glutamine, vitamin C, and linoleic acid in spinach play an important role in wound healing by increasing the formation of granulation tissues including increasing the proliferation of fibroblasts. Chitosan-collagen dressings can also increase the formation of granulation tissue. Increased proliferation of fibroblasts will increase extra cellular matrix synthesis so that the wound heals quicker. The purpose of this research is to explain the effect of merging Spinach-Chitosan-Collagen (Bakiko) as membrane form on total fibroblas in healing process of second degree burns. </em></pre><pre><strong><em>Methods:</em></strong><em> This research is true experimental laboratories with posttest only control group design. The samples were 27 Rattus Novergicus divided into 9 groups: 3 negative control group (without therapy), 3 positive control group (Bioplacenton), and 3 treatment groups (Bakiko membrane). Second degree burns made by contacting the skin to hot metal plates. Bioplacenton was applied daily on wounds while Bakiko membrane is attached to the wound and replaced every 3 days. The tissue retrieval was done after the termination of the animals on the 3rd, 7th, and 21st days post-burn induction. The tissue was made as histopathologic preparations with HE staining then calculated the total fibroblast cells using imageJ software. Data analysis was done using One Way Anova for homogeneous data and Kruskal Wallis for non homogeneous data. </em></pre><pre><strong><em>Result:</em></strong><em> One Way Anova and Kruskal Wallis test showed significant results on the 3rd day (p = 0,000) and 7th day (p = 0.004). </em></pre><strong><em>Conclussion:</em></strong><em> There is an effect of Bakiko membrane (spinach- chitosan- collagen) on the total fibroblasts in second degree burns by increasing the number of fibroblast cells at 3rd and 7th day on the proliferation phase of the burn-healing process.</em>