The effect of apoptosis induction by proteasom inhibitor MG132 combined with cisplatin in lung adenocarcinoma cell line A549

Abstract

Objective To observe the change of the expression of the apoptosis-related factor Caspase-9 and nuclear factor-κB (NF-＜B) of proteasome inhibitor MG132 combined with cisplatin (DDP) on lung adneocarinoma cell line A549.Methods Inhibition rates of the lung adenocarcinoma cell line A549 intervened by 30 μmol/L MG132 and/or 20 mg/L DDP were tested by cell counting Kit-8 (CCK-8).The morphological changes of cells were detected by Hochest33342 staining.The cells apoptosis rates were examined by flow cytometry.The changes of expression of the apoptosis-related factors Caspase-9 and NF-κB were measured by Western blotting analysis.Results CCK-8 showed that the MG132 or DDP can effectively inhibit the proliferation of cells in a dose and time-dependent manner.The flow cytometry (FCM) showed that the apoptosis rate of cells treated with MG132 and DDP (65.43 ± 1.09)％ was obviously increased than that alonely treated with MG132 (23.23 ± 0.96)％ or DDP (24.86 ± 0.40)％.Compared with the group treated MG132 or DDP,The expression of the apoptosis-related factor Caspase-9 of the combining group was significantly up-regulated and the expression of NF-κB wa significantly decreased.Conclusion MG132 combined with DDP can apparently increased the apoptosis rate of lung adneocarinoma cell line A549 through decreasing the expression of NF-κB and enhancing the expression of Caspase-9. Key words: Lung adenocarcinoma cell line A549; MG132; Cisplatin; Nuclear factor-kappa B