Effects of corilagin on proliferation of human lung adenocarcinoma cells and Notch signal pathway

Abstract

Objective To study the killing effects of corilagin on human lung adenocarcinoma cell line A549.Methods Cell culture,methyl thiazol tetrazolium (MTT) assay,Western blotting and flow cytometry were used to detect the cell cycle,apoptosis,Notch signaling pathway proteins of human lung adenocarcinoma cell line A549 treated with corilagin.Results 35 μmol/L corilagin could inhibit the proliferation of human lung adenocarcinoma cell line A549.20 μmol/L corilagin increased early apoptosis from 2.0％ to 5.4％,and late apoptosis from 3.1％ to 12.8％.40 μmol/L corilagin increased early apoptosis to 7.0％ and late apoptosis to 13.7％.Corilagin could inhibit the expression of Notch1,NICD and mammalian target of rapamycin (mTOR) protein in A549 cells.Conclusion Corilagin could inhibit the proliferation of human lung adenocarcinoma cell line A549 and induce apoptosis possibly through the Notch signaling pathway. Key words: Corilagin; Lung adenocarcinoma; Notch signaling pathway