Abstract
Background: Recently, the development new treatments and treatment combinations for advanced lung cancer have prolonged the overall survival of patients. Some chemotherapy induce bronchial infection and/or pneumonia. It is therefore necessary to understand the side effects of chemotherapy on the mucociliary clearance to reduce and predict these side effects. The bronchial beating cilia are a key apparatus to drive mucociliary clearance. Previously, we have reported the importance of ciliary beat frequency (CBF) and ciliary bend amplitude (CBA) in the evaluation of airway ciliary activities. Objectives: We investigated the influence of anticancer agents on the activity of airway beating ciliary cells (CBA and CBF) in mice. Methods: The CBF and CBA were measured using a light microscope equipped with a high-speed camera by isolating the bronchiolar ciliary cells from the mice. Various anticancer agents (docetaxel, paclitaxel and afatinib) were applied to isolated ciliary cells for 20 minutes, and the effect on the ciliary activity was investigated. Results: Docetaxel decreased CBF by 10.9 ± 2.0% and CBA by 13.3 ± 1.0% in 20 minutes. Paclitaxel decreased CBF and CBA by a similar amount. On the other hand, afatinib did not induce any change in CBA or CBF. We will also report the mechanism by which Taxan anticancer drugs cause a decrease in the activity of airway beating ciliary cells. Conclusions: The present study suggests that short-time exposure of Taxan anticancer drugs affects bronchiolar ciliary activity in a negative way. These findings suggest a need to review the management of cancer treatment-associated airway infections from the perspective of mucociliary clearance.
Published Version
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