The effect of an organomineral chelate complex on the amylolytic activity of intestinal enzymes of some fish species

Abstract

To date, there is a large amount of information regarding the effect of heavy metals on the hydrolases of the digestive tract. It has been established that even biogenic metals in high concentrations can have a toxic effect. The assessment of the activity of digestive hydrolases is relevant, since a change in their activity has a direct effect on the effectiveness of assimilation of food. Amylolytic hydrolases help to provide the body with carbohydrates (along with fats) – the main sources of energy for fish. A decrease in the intake of carbohydrates will lead to a violation of metabolic processes and, as a result, underdevelopment and death of fish. The aim of the study was to determine the effect of an organomineral chelate complex on the amylolytic activity of intestinal enzymes of some fish species. The research was conducted at the Department of Biochemistry and Physiology of the St. Petersburg State University of Veterinary Medicine and on the basis of two fisheries in the Leningrad Region. The fish of the experimental groups (n = 10) were given an additive for 30 days, the data were compared with the control (n = 10). The drug was given together with the feed once a day, at a concentration of 0.5 g / kg (feed weight). Fingerlings (0+) of 3 species of freshwater bony fish were used as research objects: Carassius auratus (n=20), Cyprinus carpio (n=20), Oncorhynchus mykiss (n=20). Amylolytic activity was determined in the total homogenets of the intestinal mucosa of fish. The activity was assessed by the increase in hexoses by the Nelson method in the modification of Ugolev, Jesuitova. The detection was carried out on a photoelectric KKK-3 photometer. Based on the studies obtained, it can be concluded that the use of an organomineral chelate complex in the studied dosage (previously worked out in conditions of determining acute and chronic toxicity for fish) does not have a negative effect on the amylolytic activity of intestinal enzymes. There was no significant decrease in amylolytic activity in all experimental groups. Based on this, it is possible to recommend the use of a chelate complex for fish in industrial aquaculture.