Expression of cytochrome genes during the early gonadal development in triploid female rainbow trout Oncorhynchus mykiss

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 三倍体雌性虹鳟性腺发育阶段细胞色素相关基因的表达 DOI: 作者: 作者单位: 1. 上海海洋大学 水产科学国家级实验教学示范中心, 上海 201306;2. 中国水产科学研究院黑龙江水产研究所, 黑龙江 哈尔滨 150070;3. 上海海洋大学 水产动物遗传育种中心上海市协同创新中心, 上海 201306 作者简介: 相福生(1990-),男,硕士研究生,主要从事冷水鱼育种与繁殖的研究.E-mail:fishxiangfs@163.com 通讯作者: 中图分类号: S917 基金项目: 现代农业产业技术体系专项资金项目（CARS-46）；中国水产科学研究院基本科研业务费项目（2018HY-ZD03）；黑龙江省自然科学基金面上项目（C2016069）. Expression of cytochrome genes during the early gonadal development in triploid female rainbow trout Oncorhynchus mykiss Author: Affiliation: 1. National Demonstration Center for Experimental Fisheries Science Education;Shanghai Ocean University, Shanghai 201306, China;2. Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;3. Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding;Shanghai Ocean University, Shanghai 201306, China Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:等细胞色素相关基因能够调节硬骨鱼类性类固醇的合成，对性腺发育和性别决定产生影响。本研究以全雌三倍体虹鳟（）为研究对象，正常雌性二倍体虹鳟为对照，选取31~68 dpf（days post fertilization）时间段的虹鳟仔鱼脑组织，采用qRT-PCR和酶联免疫的方法研究以上几种基因的表达状况和脑芳香化酶的活性变化，以期探明导致三倍体雌性虹鳟性腺发育异常的关键原因。qRT-PCR结果显示，二倍体中在30~50 dpf时表达量上调并且维持在较稳定水平，但50~56 dpf时表达量逐渐下调，之后56~68 dpf表达量持续上调；三倍体中表达量在30~35 dpf开始上调，35~47 dpf逐渐下调，47~55 dpf开始第二次上调，之后维持在较稳定水平直至68 dpf，但三倍体表达量在34 dpf出现峰值，三倍体表达量在33~42 dpf时维持在较高水平，在38 dpf时出现高峰；三倍体在37 dpf出现峰值，之后开始下调；三倍体在40 dpf出现峰值，之后逐渐下调，但三倍体的表达量在35~46 dpf时逐渐上升，在45 dpf时达到高峰之后直至69 dpf逐渐下降，并且维持在较为平稳的水平上；但是在相同的实验条件下未检测到同一时期三倍体P<0.05）高于三倍体虹鳟，尤其在45~50 dpf时，该酶活性分别较三倍体的高1.15倍和1.12倍。以上结果表明三倍体虹鳟早期性腺发育迟缓的原因之一是等基因的表达晚于二倍体，且表达量低于二倍体，造成雌二醇不能正常合成，最终导致性腺发育迟缓。 Abstract:The genes play an important role in the steroidogenesis and gonadal differentiation of teleosts. Therefore, in this study, we chose triploid and diploid rainbow trouts () at 31-68 days post fertilization (dpf) cultured in the same environment as object. In this study, triploid rainbow trouts were used as object and diploid rainbow trouts were used as controls to evaluate the expression of genes and the activity of CYP19A1B enzyme in the brain of rainbow trout. The real-time polymerase chain reaction results showed that the expression of in the diploid rainbow trout was up-regulated during 30-50 dpf and maintained a stable level during this period. Subsequently, during 50-56 dpf, the expression was down-regulated; during 56-68 dpf, the expression was up-regulated again. The expression of in the triploid rainbow trout was up-regulated during 30-35 dpf, down-regulated during 35-47 dpf, and then upregulated during 47-55 dpf; thereafter, until 68 dpf, the expression was maintained at a stable level. The expression of in diploid rainbow trout peaked at 34 dpf and in triploid rainbow trout peaked at 38 dpf. The expression of in the diploid rainbow trout was maintained at a high level during 33-42 dpf and its expression peaked at 38 dpf. The expression of in the triploid rainbow trout was high during 47-59 dpf and peaked at 49 dpf. The expression of in diploid rainbow trout peaked at 37 dpf, and then the expression was down-regulated. In the triploid rainbow trout, the peak value was recorded at 40 dpf, and then the expression was down-regulated, but the expression of in the triploid rainbow trout was lower than that in the diploid rainbow at the same period. The expression of in the diploid rainbow trout was up-regulated at 35-46 dpf and the peak value was recorded at 45 dpf, and then the expression was down-regulated until 69 dpf. However, under the same experimental conditions, the expression of in the triploid rainbow trout was not detected. The results of enzyme-linked immunosorbent assay demonstrated that at 40 dpf, the activity of CYP19A1B enzyme in the diploid and triploid rainbow trout peaked, but during 40-60 dpf, the activity of CYP19A1B enzyme in the diploid rainbow trout was significantly higher than that in the triploid rainbow trout; especially at 45 dpf and 50 dpf, the activity of this enzyme in the diploid trout was 1.15 and 1.12 times higher than that in the triploid trout, respectively. The results suggest that one of the reasons for early gonadal differentiation delay in triploid rainbow trout is the expression of is lower and later than those in the diploid rainbow trout. Moreover, estradiol cannot be synthesized normally in triploid rainbow trout, and therefore, gonadal differentiation is delayed. 参考文献 相似文献 引证文献