The effect of amino acids on porcin oocytes IVM and reconstructed embryonic early development

Abstract

The purpose of this study is to compare the effect of non-essential amino acids and essential amino acids on in vitro maturation and development of reconstructed embryos of porcine oocytes. The oocytes were divided into four groups, the IVM medium of porcine were added 1% the essential amino acids, 1% non-essential amino acids, both 1%essential and nonessential amino acids, And non amino acids as control. The oocytes were cultured for 44h, and statistics maturation rate. Embryo culture medium (NCSU-23) were added 1% the essential amino acids, 1% non-essential amino acids, both 1%essential and nonessential amino acids, And free amino acids as control. The reconstructed embryos were cultured to 7d, and statistics blastocysts rate. Each experiment was repeated three times. The results show that added any kind of amino acids in IVM medium will improve the oocytes maturation rate (P<;0.05), and the oocytes maturation rate of non-essential amino acid group was significantly higher than other groups (P<;0.05); The non-essential amino acids in NCSU-23 can significantly improve the reconstructed embryonic blastocysts rate (P<;0.05), while the essential amino acids or both essential and non essential amino acids in NCSU-23 leads blastocysts rate decreased (P<;0.05). The studies have shown that there are some different of the effect of essential and non essential amino acids on porcine oocytes IVM and reconstructed embryos development.