The effect of added organic matter on the soil formation of oil-based drill cutting pyrolysis residue.

Abstract

In order to explore the effects of added organic matters on the soil formation process of oil-based drill cutting pyrolysis residue, two experiment groups (pure and adding) were studied to indicate the soil microbial community and the function of nitrogen cycling. Samples were collected in the third and fourth years based on the plant growth. The 16S rRNA high-throughput sequencing technology and PICRUSt were used to study microbial communities and the function of nitrogen cycling. The results showed that MND1 (2.5-4.5%), Sphingomonas (1.4-2.4%), and other typical oil-degrading bacteria were discovered. The diversity of the microbial community decreased with the duration of the experiment. However, it was higher than that of the background soil. The relative abundance of microbial nitrogen dissimilation reduction (0.9-1.0‰) was the highest among the two experiment groups and the background soil. The second and third were denitrification and nitrogen fixation. Nitrification gene abundance was the lowest among the five main steps of nitrogen cycling. The addition of organic matters could improve soil formation of pyrolysis residues. This study provides scientific data for pyrolysis residue to restore soil functions.