The effect of 2-oxoindoline-3-glyoxylic acid derivatives on animals’ behavioral and autonomic reactions in the open field test

Abstract

ABSTRACT Screening research on the effect of 2-oxoindoline-3-glyoxylic acid derivatives on the emotional and behavioral responses of rats in the open field test was performed. 2-oxoindoline derivatives were suspended in the injection water using the Tween-80 emulsifier (one drop with 25 mg of test substance) and intraperitoneally administered at a dose of 12 mg/kg 1 hour before the first test. Diazepam was administered in the dosage of 2 mg/kg and in the same way as the investigated compounds. Neuroethological indicators have been studied including the latent period of the first movement, ambulation (horizontal activity), number of upright postures (vertical activity), the number of times animals moved to the center, autonomic balance indicators such as the number of grooming acts and defecation acts calculated by the number of fecal balls (boluses). It was found that most 2-oxoindoline derivatives suppressed or did not affect animals’ orienting and investigatory responses in the open field test. Under these conditions, the compounds suppressed emotionality manifested as a reduced number of fecal balls and grooming acts indicating a decrease in anxiety and fear in a stressful situation caused by the novelty of the open field test. Based on the analysis, it can be argued that 2-oxoindoline derivatives can modify the psychophysiological features of intact rats, i.e. their neuropsychotropic properties.