Abstract
Introduction: Dental caries is a multifactorial disease caused by the interaction of pathogenic bacteria, especially Streptococcus mutans. Various caries prevention chemically and mechanically can help reduce the number of bacteria. However, this approach does not cover the entire bacterial population with a photodynamic therapy approach with the addition of photosensitizers and low-intensity light radiation with the right wavelength able to eliminate the number of S. mutans bacteria. Purpose:To determinethe duration of effective irradiation time on a 405 nm diode laser with erythrosine photosensitizer in killing S. mutans bacteria.Methods: This research was an experimental laboratory type with 25 S. mutans samples divided into 5 groups treated with erythrosine photosensitizer 0.1 mg / ml and 405 nm diode laser irradiation with 45 seconds, 60 seconds, 75 seconds duration and no erythrosine photosensitizers and without irradiation. The growth of S. mutans bacteria was calculated then the data were analyzed statistically.Results:the research found that S. mutans bacteria decreased gradually in each group; erythrosine without photosensitizer and without irradiation had the highest average growth rate of 71.6 CFU / ml; without photosensitizer with irradiation of 40.6 CFU / ml; irradiation with photosensitizer 45 seconds at 20.6 CFU / ml; irradiation with 60 seconds photosensitizer at 11 CFU / ml; and irradiation with 75 seconds photosensitizer at 0 CFU / ml. In statistical tests, the data are normally distributed and homogeneous. There are significant differences between groups.Conclusion: The effective duration in this study was found at 75 seconds.
Highlights
Dental caries is a multifactorial infectious disease as the result of pathogen bacteria interaction, especially Streptococcus mutans in oral colonization
An approach is needed which can provide the possibility of reducing oral bacteria number efficiently with minimum damage to dental health and secondary caries formation prevention which reduces the possibility of material substitution and pulp inflammation
Based on the existence of various opinions about the advantage of photodynamic therapy using erythrosine photosensitizer material. and based on the research obtained by the effective laser irradiation time of the diode with chlorophyll and methylene blue photosensitisers at 75 seconds
Summary
Dental caries is a multifactorial infectious disease as the result of pathogen bacteria interaction, especially Streptococcus mutans in oral colonization. Photodynamic therapy provides new modalities to reduce pathogenic bacteria and prevent new dental caries lesions. A research by (Araujo Neto et al, 2017)[4] reported that the usage of photodynamic therapy with methylene blue photosensitizer for 90 seconds, significantly could decrease the number of Streptococcus mutans.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
