Abstract
We report the draft genome sequence of Actinokineospora bangkokensis 44EHWT, the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain.
Highlights
Generation sequencing and genome mining are powerful and rapid technologies to identify the genetic potential of a strain to synthesize secondary metabolites with various biological activities
Thailandin B is probably the precursor of the A-form, because thailandin A is further rhamnosylated. Both compounds show activity against pathogenic fungal strains with minimum inhibitory3.concentrations ranging between 16–32 μg/mL [8]
T produces the polyene thailandin and plasma bangkokensis membranethailandin by interacting withB isthe mainthesterol, ergosterol, which results in membrane
Summary
Generation sequencing and genome mining are powerful and rapid technologies to identify the genetic potential of a strain to synthesize secondary metabolites with various biological activities. One order known to produce many secondary metabolites with different bioactivities is the Actinomycetales. Under laboratory conditions only a few compounds are produced by a strain while their genomes comprise often more than 20 biosynthetic gene clusters. Cryptic clusters have been activated by heterologous expression [1], changing growth conditions [2] or by the manipulation of regulatory genes [3,4]. The knowledge of the genome sequence and the biosynthetic cluster composition of a secondary metabolite gives insights into the biosynthetic pathway. It is a valuable tool for metabolic engineering to increase the production of a specific compound or to generate novel metabolites by combinatorial biosynthesis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
