Abstract
Poly(A)-containing cytoplasmic RNA and nuclear RNA from Rous sarcoma virus (RSV)-induced hamster tumor cells was fractionated on sucrose density gradients and hybridized with a [ 3H]cDNA probe to determine the size distribution of virus-specific RNA. One size class of viral RNA sedimenting at 21 S was detected in cytoplasmic poly(A)-containing RNA. Nuclear RNA contained three species of virus-specific RNA sedimenting at 47, 35, and 21 S. Sedimentation of nuclear RNA under denaturing conditions did not change this size distribution.
Published Version
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