The distribution of orthogonal arrays in the freeze-fractured rat median eminence.

Abstract

The distribution of orthogonal arrays of particles and their relationships to gap and tight junctions have been studied in the glia of the freeze-fractured rat median eminence (ME). These rectilinear clusters of intramembrane particles are thought to represent trans-membrane channels for ions or metabolites, and were found to be densely packed on the membranous laminations of the pial-glial limitans. Additionally, arrays were found to be present on all of the perivascular glial end-feet examined. Two classes of end-feet were distinguished by their relative densities of orthogonal arrays. End-feet displaying low densities of arrays occurred more frequently in the internal zone, while end-feet displaying high densities occurred more often in the external zone. Similar distinctions based on array density could be made in membranes from other regions of the cell as well. Cross-fractures revealing the cytoplasm underlying these membranes often exposed lipid inclusion bodies, suggesting that membranes containing few arrays belong to tanycytes (or to 'astrocyte-like tanycytes'). The distribution of arrays appeared to be unrelated to the distribution of gap junctions in the membranes of astrocytes and tanycytes (and 'astrocyte-like tanycytes') of the ME, appearing near to and far from gap junctions with approximately equal frequency. Orthogonal arrays were absent from glial membranes near synaptic profiles in the ME. Arrays were also absent from the microvillous membranes of the apical surfaces of ependymal cells, from the cytoplasmic protrusions into the CSF of tanycytes, and from the vicinity of the tight and complex junctions linking the tanycyte and ependymal cell lateral membranes near their apical poles. These results suggest that there is a gradient of array density for most glia of the ME, increasing from the ventricular to the pial surface.