Abstract
The distribution of nitrate reductase (NR, EC 1.6.6.1.) in the leaves of single‐stem tomato plants (Lycopersicon esculentum Mill., cv. Vandenbergs Moneydor) was studied using an in vitro test. The activity decreased from young to old leaves. However, a low value (NR minimum) occurred in some leaves below the apex, usually in the almost completely expanded leaves, provided that the plants received sufficient nitrate to induce optimum NR activity in all the leaves. When insufficient nitrate was available there was NR in the young leaves only. The observed NR minimum coincided with a low value for soluble carbohydrates and amino acids. Since there was no extra export of labelled carbon from the leaves with the NR minimum, it is suggested that in the almost completely expanded leaves carbohydrates produced by photosynthesis are mainly used for the production of polysaccharides for new cell walls. Consequently, less are left for the production of keto acids, which can act as acceptors for reduced nitrogen. Therefore, less amino acids are produced, and this may result in a lowered protein synthesis, including a lowered synthesis of nitrate reductase.
Published Version
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