Abstract
Numerous experiments have demonstrated that skin has a profound influence on the pattern of limb regeneration in urodeles. In this investigation, the fate during regeneration of marked cells derived from narrow strips of skin inserted into different positions around the limb circumference has been followed. Skin strips were taken from triploid axolotls and transplanted into diploid sibling animals. The distribution of trinucleolate cells was determined at the site of amputation and in the regenerated limb. The results indicate that at the time of amputation marked cells appear to be localized to the graft, whereas in the regenerate marked cells may be found at all proximal-distal levels and at any position around the circumference of the limb. These results are discussed in terms of a possible mechanism for distal outgrowth.
Highlights
Numerous experiments have shown that the skin of the amphibian limb stump can influence the pattern of the regenerate
In contrast to the distribution of marked cells in the regenerates, grafted cells are significantly localized in the amputated segment of the limbs (Fig. 4), where the majority of the cells with three nucleoli are found in quadrants 45 and 315, that is, in the quadrants containing the graft. This experiment has shown that a narrow strip of skin from the upper arm of a triploid axolotl, when transplanted into the upper arm of a diploid axolotl, influences the pattern of the regenerate after amputation through the grafted region in the same manner as previously reported for the newt (Rollman-Dinsmore and Bryant, 1982)
When the skin graft is transplanted to a site 180” opposite to its original site and the limb is subsequently amputated through the graft, the ensuing regenerate forms supernumerary digits in a majority of the cases
Summary
Numerous experiments have shown that the skin of the amphibian limb stump can influence the pattern of the regenerate. Even a small strip of skin added to the limb circumference may produce these results (RollmanDinsmore and Bryant, 1982; Tank, 1981) The formation of these abnormal regenerates is dependent on grafting so as to appose cells of disparate circumferential positional values. Various experiments have shown the effect of skin on patterning, only Slack (1980, 1983) has reported on the fate of skin cells from surgically constructed limbs after regeneration. He reported that cells from triploid skin grafts remain localized in structures adjacent to the graft. The data obtained indicate that contrary to Slack’s results, marked cells in the regenerates were not localized to the region adjacent to the graft, but were found distributed around the circumference of the regenerate
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
