The distinct enzymic lipid peroxidation systems from liver microsomes in the presence of ADP— or EDTA—iron complexes

Abstract

It is known that the ADP-Fe complex is the most effective and widely used cofactor for lipid peroxidation in rat liver microsomes [ 1,2] . Many authors have considered the iron ions as initiators of this freeradical reaction [3,4] and ADP as a component which prevents the precipitation of iron at neutral pH [4]. However, the latest data on the reconstruction of the microsomal lipid peroxidation system indicate that besides ADP-Fe the presence of the EDTA-Fe complex is also necessary [5]. In this report data are presented about the interchange ability of ADP-Fe and EDTA-Fe in NADPHdependent lipid peroxidation in intact rat liver microsomes.