Abstract
Cannabidiol (CBD), as the only phytocannabinoid that has no psychoactive effect, has both antioxidant and anti-inflammatory effects, and thus might be suggested as a cytoprotective compound against UV-induced metabolic changes in skin cells. Therefore, the aim of this study was to investigate the level of protective CBD activity by evaluating the proteomic profile of 2D and 3D cultured skin fibroblasts models following exposure to UVA and UVB radiation. The CBD cytoprotective effect against UV-induced damage in 2D and 3D cultured fibroblasts were different. The main alterations focus on the range of cell reaction and involved different proteins associated with various molecular functions. In the 2D cultured cells, following UV radiation, the major changes were associated with proteins involved in antioxidant response and inflammation, while, in the 3D cultured fibroblasts, CBD action against UV induced changes were mainly associated with the activation of signalling pathways. Therefore, the knowledge of the CBD action in a multilayer skin cells model allowed for the prediction of changes in cell-cell interactions and skin cell metabolism. Knowledge about the lower protective effect of CBD in 3D cultured fibroblasts should be taken into account during the design of UV light protection.
Highlights
Cannabidiol (CBD) is a major, biologically active, phytocannabinoid that has recently become increasingly used in cytoprotection [1,2]
It has been previously shown that cells in 3D culture differ in proteomic profile from 2D cultured cells mainly in terms of proteins that are the part of a functional network including structural proteins, chaperones, as well as oxidative stress-related proteins [17]
CBD-treated 2D cultured cells exposed to UV-radiation exhibited a more marked effect on the levels of proteins involved with antioxidant response and inflammation
Summary
Cannabidiol (CBD) is a major, biologically active, phytocannabinoid that has recently become increasingly used in cytoprotection [1,2]. Scavenger and as an antioxidant in cells under stress conditions [5]. CBD anti-apoptotic activity has been previously described, showing that CDB has a positive effect on cell survival [6]. CBD may show protective actions against UV-induced oxidative stress in skin cells exposed to sunlight. The UVA and UVB regions of the spectrum reaching the human skin have damaging effects which influence the cell function by direct formation of ROS and modifying cellular compounds such as proteins, lipids and nucleic acids [7]. Changes in cellular metabolism are observed when skin cells are exposed to UV radiation, leading to impaired skin condition and functioning [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
