The diagnostic value of VILIP-1 and NR2 peptide combined detection in the early ischemic stroke

Abstract

Objective To Investigate the concentration of VILIP-1 and NR2 peptide in the serum of patients with ischemic stroke,and to explore their clinic value in early diagnostic of ischemic stroke patients. Methods The levels of VILIP-1 and NR2 peptide were examined by ELISA (enzyme linked immunosorbent assay,ELISA) with suspicious TIA (defined as a neurological deficit that resolved within 24 hours) or acute ischemic stroke patients (within 72 hours of onset of symptoms) 340 cases,102 healthy controls,98 patients with vascular risk factors and 35 patients with hemorrhagic stroke.Among all the groups,VILIP-1 and NR2 peptide level were analyzed using the nonparametric Wilcoxon test.Diagnostic performance were analyzed among the groups with the two biomarkers independently and combinedly. Results Serum levels of VILIP-1 and NR2 peptide in patients with ischemic stroke (IS) were 9.80 (1.90-14.22) μg/L,14.40 (5.60-27.91) μg/L respectively，which was higher than that of the healthy control group ［VILIP-1：0.02 (0.01-0.09)，NR2：0.33 (0.02-1.15)，χ2 were 5.61 and 9.54，P<0.001］,the group with vascular risk factors ［VILIP-1：0.03 (0.02-0.16),NR2：0.27 (0.01-1.54),χ2 were 6.74 and 10.62，P<0.001］,the group of patients non-stoke ［VILIP-1：0.04 (0.03-0.19)，NR2：0.53 (0.45- 1.21)，χ2 were 3.78 and 7.63，P<0.001］.The levels of VILIP-1 and NR2 peptide was significantly increased in IS patients presenting within 3 h of symptom onset.When differentiating IS from patients with hemorrhagic stroke,NR2 had a AUC of 0.934,showing a strong distinguishing effectiveness.Differentiating IS from healthy controls,patients with vascular risk factors and non-stroke patients,the AUC of combination of VILIP-1 and NR2 was 0.974,which was higher than the AUC of either VILIP-1(0.849) or NR2(0.862) alone（P<0.05）. Conclusions VILIP-1 and NR2 peptide are very sensitive and specific biomarkers to the early diagnosis of IS.The combination of VILIP-1 and NR2 peptide has higher value of clinical applications than one of them independently.（Chin J Lab Med,2014,37:469-472） Key words: Brain ischemia; Stroke; Neurocalcin; Receptors,N-methyl-D-aspartate