Abstract
This article describes the development of the experimental setup for measuring the cell membrane electrical potential by Double -Sucrose-Gap Technique. The double-gap isolation method allows the simultaneous measurement of electrical activity and tension output from contracting segments of muscle fibers. This technique has been widely used as a convenient tool for recording of the membrane activities from myelinated or unmyelinated nerves and muscle preparations. This device can be an effective way to provide undergraduate biomedical engineering students with invaluable experiences in neurophysiology. The installation design and its main characteristics are described. The advantages of the described device are the simplicity of the experiment, relatively low cost, the possibility of long-term experiment.
Highlights
The study of the various cells in the body is a very important aspect in the investigation of any tissue, organ or organism as a whole, because the actions of cells underlie responses of vast majority of biological systems
This paper examines the basic principles of the cell membrane potential registration by double sucrose gap method and describes the installation design and its main characteristics
There are a lot of electrochemical processes in living cells, but most clearly they are expressed on the cell membrane surface, which separates the inner cell volume from of the intercellular substance and other cells
Summary
The study of the various cells in the body is a very important aspect in the investigation of any tissue, organ or organism as a whole, because the actions of cells underlie responses of vast majority of biological systems. The main advantages of the method are due to the fact that experimenter does not operate with a single cell, but with a tissue fragment, for example, muscle or nerve fiber [1]. This greatly simplifies experimentation and reduces the cost of the equipment, because there is no need to use microelectrodes. The devices of this type are not produced in Russia Devices of this type can be custom-made, they are very expensive and designed to work with the nervous tissue, do not allow to obtain data about the relationship between contractile and electrical activity of the investigated muscle tissue.
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