Abstract
The Elaeagnus L. species are trees and bushes that mainly grow in temperate zones of Western Europe; Minor, Central, and Southeast Asia; the Far East; and North America. Some species are used as fruit or ornamental plants and have economic value. Problems with the identification of species in the Elaeagnus genus by molecular genetical methods arise in the study of populations, systematics, breeding, and other areas of plant science and practice. Recently, the polymorphism of 5S ribosomal DNA non-transcribed spacers (5S rDNA NTSs) in Elaeagnaceae Adans. has been described. The results were used in our study as a basis for development of new species-specific molecular markers for some members of the Elaeagnus genus. The author’s method was applied for finding regions that were potentially applicable for species-specific primer design. As a result, some species-specific molecular markers were developed for Elaeagnus angustifolia L., E. commutata Bernh., E. pungens Thunb., and E. multiflora Thunb. These markers were tested in a range of samples and showed the presence of amplified fragments in lanes of the marked species only. Samples of other species showed no amplifications. Thus, the developed markers may be useful for the species identification of the studied Elaeagnus plants in botanical, dendrological, and genetic research (especially in a leafless period of year), as well as in breeding and hybridization experiments.
Highlights
The Elaeagnaceae Adans. family consists of three genera: Elaeagnus L., Hippophae L., and Shepherdia Nutt. [1]
Some species-specific molecular markers were developed for Elaeagnus angustifolia L., E. commutata Bernh., E. pungens Thunb., and E. multiflora Thunb
Twelve alignments of E. angustifolia, E. commutata, E. pungens, and E. multiflora Non-Transcribed Spacers (NTSs) were obtained for the search of high polymorphic decinucleotide fragments, which could be a basis for species-specific marker development (Supplementary Materials Tables S1–S12)
Summary
The Elaeagnaceae Adans. family consists of three genera: Elaeagnus L., Hippophae L., and Shepherdia Nutt. [1]. In terms of the number of species, Elaeagnus is superior to the rest It comprises nearly 100 species, which are trees and bushes that mainly grow in temperate zones of Western Europe; Minor, Central, and Southeast Asia; the Far East; and North America. These plants have shoots and leaves that are tightly covered with scales or hairs, and the plants appear to have a silvery green color. Such species as Elaeagnus angustifolia L., E. commutata Bernh., E. pungens Thunb., E. umbellata Thunb., and E. multiflora Thunb. The fruit of E. angustifolia, E. multiflora, and E. umbellata are widely used for food [4]. Associated with this, genetic investigations and the development of molecular approaches in Elaeagnus species identification are especially needed
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