Abstract

ABSTRACT An in vitro system for investigating the effects of steroid hormones and protein synthesis inhibitors on hypothalamic peptidases inactivating oxytocin has been developed. In the presence of oestradiol-17β enzyme activity was increased in the in vitro system whilst this increase was blocked completely by cycloheximide and partially blocked by actinomycin-D. It is apparent therefore that oestradiol-17β acts directly on the hypothalamus stimulating oxytocinase activity.

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