The development of allergic inflammation in a murine house dust mite asthma model is suppressed by synbiotic mixtures of non-digestible oligosaccharides and Bifidobacterium breve M-16V.

K A T Verheijden,G Folkerts,T Leusink-Muis,A D Kraneveld,P V Jeurink,J Garssen,S Braber,L E M Willemsen

doi:10.1007/s00394-015-0928-8

K A T Verheijden, G Folkerts + Show 6 more

Open Access

https://doi.org/10.1007/s00394-015-0928-8

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Abstract

Purpose The incidence and severity of allergic asthma is rising, and novel strategies to prevent or treat this disease are needed. This study investigated the effects of different mixtures of non-digestible oligosaccharides combined with Bifidobacterium breve M-16V (BB) on the development of allergic airway inflammation in an animal model for house dust mite (HDM)-induced allergic asthma.MethodsBALB/c mice were sensitized intranasally (i.n.) with HDM and subsequently challenged (i.n.) with PBS or HDM while being fed diets containing different oligosaccharide mixtures in combination with BB or an isocaloric identical control diet. Bronchoalveolar lavage fluid (BALF) inflammatory cell influx, chemokine and cytokine concentrations in lung homogenates and supernatants of ex vivo HDM-restimulated lung cells were analyzed.ResultsThe HDM-induced influx of eosinophils and lymphocytes was reduced by the diet containing the short-chain and long-chain fructo-oligosaccharides and BB (FFBB). In addition to the HDM-induced cell influx, concentrations of IL-33, CCL17, CCL22, IL-6, IL-13 and IL-5 were increased in supernatants of lung homogenates or BALF and IL-4, IFN-γ and IL-10 were increased in restimulated lung cell suspensions of HDM-allergic mice. The diet containing FFBB reduced IL-6, IFN-γ, IL-4 and IL-10 concentrations, whereas the combination of galacto-oligosaccharides and long-chain fructo-oligosaccharides with BB was less potent in this model.ConclusionThese findings show that synbiotic dietary supplementation can affect respiratory allergic inflammation induced by HDM. The combination of FFBB was most effective in the prevention of HDM-induced airway inflammation in mice.

Highlights

Allergic asthma is a chronic disease that affects around 235 million people worldwide
Airway hyperresponsiveness (AHR) to methacholine aerosols was measured at baseline; no differences were observed between the PBS- and House dust mite (HDM)-challenged mice fed the different diets (Fig. 1b)
The AHR of HDM-challenged mice fed GFBB or FFBB remained below the AHR of HDM-challenged mice fed the control diet albeit this did not reach significance (Fig. 1c)

Summary

Introduction

Allergic asthma is a chronic disease that affects around 235 million people worldwide. The activated DCs take up the HDM allergen and induce the development of naïve T cells into antigen-specific Th2 cells in the local lymph nodes These Th2 cells migrate back to the lung mucosal tissue. We have shown that GOS alone are capable of suppressing HDM-induced airway hyperresponsiveness, airway eosinophilia and Th2-related cytokine and chemokine concentrations in the lung [16]. The combination of GFBB or FFBB with acidic oligosaccharides reduced allergic responses in food allergic mice and suppressed airway inflammation in a mouse model for ovalbumin-induced asthma, respectively [17, 18]. The aim of this study was to investigate the effect of two different synbiotic mixtures, GFBB and FFBB, on pulmonary inflammation, cytokine and chemokine concentrations in lungs and BALF of HDM-induced asthmatic mice

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