Abstract
A screening process has been developed to evaluate four dietary supplements for their potential to affect coagulation through the extrinsic pathway in human tissue. An established analytical method was used to determine prothrombin time (PT) using a clinical laboratory fibrometer.1,2 Dong quai, Korean ginseng, cat's claw, and ginger were studied. Extracts of plant material were made by mixing the native material in synthetic plasma at room temperature for 1.0 hour. Plasma was simulated by preparation of 6% bovine albumen, phosphate buffered at pH 7.3, with the ionic strength controlled using common physiologic salts. Concentrations of the extract were prepared by varying the amount of material extracted with a given volume of synthetic plasma. Initially, a volume study was conducted to determine the extent to which an aliquot of human plasma could be diluted before changing the PT. Ten microliters of the appropriate extract were added to an aliquot of pooled human plasma collected from male volunteers bet...
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call