The determination of bisphenols contained in food-related products with increased accuracy provided by the H-point Standard Addition Method (HPSAM)-based methodology

Abstract

The basic version of the H-point standard addition method and its variants have been developed with an increased number of measurement conditions (from two to five conditions allowed for differentiation of sensitivity). The methodology adapted to the signal increment and generalized versions was used for the determination of the harmful compounds from the group of bisphenols (bisphenol A, S, and F) using high-performance liquid chromatography with a diode array detector. This approach in theoretical and experimental studies allowed for obtaining high-quality results after eliminating all types of interference effects: multiplicative, additive, and nonlinear. The analysis of food-related samples, such as store receipts and canned food, confirmed the effectiveness of the developed methodology. The analytical results were distinguished by very good accuracy (RE<5 %, RV∼100 %) and precision (CV≤4 %). The approach can be successfully applied to various analytical systems (RGB model) and is characterized by a high greenness value (AGREE approach).